Drag Delivery System Using Microbubbles and Ultrasound

背景：近年来提出了一种基于微泡和超声的给药系统。我们评估了荧光结合微泡能否通过超声在靶组织内保留。方法：麻醉大鼠72只。制备了全氟化碳暴露的超声葡萄糖白蛋白（PESDA）微泡，并与荧光Cy^<3TM>偶联。Cy^<3TM>连续注射PESDA，并在多个超声和微泡设置下对心脏超声。提取心脏，用离线软件分析组织内的荧光强度。结果：微泡浓度对荧光强度无明显影响；而微泡注入速度、超声持续时间和声功率对超声效果有影响。结论：超声和微泡联合给药是可行的，其效果取决于微泡和超声的设置。

Background : Drug delivery system using microbubble and ultrasound is recently proposed. We evaluated whether fluorescence conjunct with microbubbles can be retained within the target tissue by ultrasound insonation.Methods : 72 anesthetized rats were used in this study. Perfluorocarbon-exposed sonicated dextrose albumin (PESDA) microbubbles were made and conjugated with Cy^<3TM>, which is fluorescence. Cy^<3TM> PESDA was continuously injected and ultrasound was insonated to the heart in several ultrasound and microbubble settings. The heart was extracted and the intensity of fluorescence within the tissue was analyzed by offline software.Results : Concentration of microbubble did not influence the intensity of fluorescence; however, injection speed of microbubble, duration of insonation, and acoustic power influenced.Conclusion : Our results suggest that drug delivery using microbubble and ultrasound is possible and the effects depend on the settings of microbubble and ultrasound.