Research on the substance in the brain involves in functional recovery by exercise after cerebral infarction

为了探讨促进脑梗塞后功能恢复的物质，我们利用光化学脑梗塞大鼠进行了以下实验。我们根据Feeney的光束行走评估来评估性能。观察2周后神经功能恢复的时间进程和GDNF表达的变化。结果：1.功能恢复在1周内明显，随着功能恢复，梗死灶周围GDNF阳性细胞增多。观察2周后步行训练组、跑台训练组和自然病程组的功能恢复情况。运动组恢复早于自然课程组，步行锻炼效果优于跑步机锻炼。这些结果表明，运动促进了机能的恢复，高级运动比单纯运动更有效。我们研究了第二次对侧梗塞后的功能恢复情况。首次脑梗塞后，大鼠在2周后基本恢复到正常水平。但在第二次对侧脑梗塞后，2周后功能恢复维持在较低水平。这些结果表明，对侧皮质在脑梗塞后的功能恢复中起重要作用。我们观察了依达拉奉对脑功能恢复、脑梗塞面积和caspase表达的影响。腹腔注射依达拉奉1周后缩小梗塞面积，促进功能恢复，注射后24小时梗死区边缘caspase阳性细胞表达增加。以上结果提示，依达拉奉可扩大半影区，缩小梗塞面积，促进功能恢复。

To investigate he substance which promotes functional recovery after cerebral infarction, we conducted following experiments using rats with dhotochemically cerebral infarction. We evaluated performance according to Feeney's beam walking evaluation.1. We investigated the time course of functional recovery and the change in the expression of GDNF for 2 weeks. Functional recovery was prominent during first 1 week and GDNF-positive cells around the infarction were increased on a parallel with functional recovery.2. We investigated differences in functional recovery among beam walking exercise group, treadmill exercise group and natural course group for 2 weeks. Exercise groups recovered earlier than natural course group and beam walking exercise was more effective than treadmill exercise. These results suggest that the exercise promotes functional recovery and advanced exercise is more effective than simple exercise.3. We investigated functional recovery following second contralateral infarction. Following first infarction, rats recovered nearly equal to normal level after 2 weeks. But following second contralateral infarction, functional recovery stayed low level after 2 weeks. These results indicate that contralateral cortex is important in functional recovery after cerebral infarction.4. We investigated the effect of edaravone on functional recovery, infarct size, and the expression of caspase. Intraperitoneal injection of edaravone for 1 weeks reduced infarct size and promoted functional recovery and the expression of caspase-positive cells in the border of infarct area increased 24 hours after injection. These results suggest that edaravone expands penumbra area leading to the reduction of infarct size and the promotion of functional recovery.