Development of Suppression and Reduction System for Toxic Cyanobacteria in Asia Pacific Region

本研究在泰国的实验池塘上，研究了沟槽种植水生植物去除营养物质和生物反应器固定捕食性微型动物的混合系统对微囊藻等有害蓝藻的抑制效果。将现场废弃的废塑料制成的纤维状载体填充到生物反应器中，并将可食用的水生植物白杨种植到渠道中。利用该复合系统对有毒微囊藻进行了长期的去除试验，结果表明，该生物反应器对有毒微囊藻的去除效率可达95%以上。在实验池中几乎没有检测到的微型动物聚集在生物反应器载体表面，密度很高，观察到大量捕食微囊藻的微型动物群落。在微型动物捕食微囊藻后，微囊藻毒素有可能残留在生物反应器中。由于采用高性价比高效液相色谱(HPLC)进行分析，从生物反应器出水中未检测到微囊藻毒素。这一结果表明，有能力产生酶，通过这种酶，微型动物或生物反应器中的细菌可以分解微囊藻毒素。尽管生物反应器的出水进入通道，但微囊藻毒素被完全分解，因此水生植物对食品的安全性得到了提高。槽内硝态氮和氨氮的平均去除效率分别为40%和30%。此外，成长起来的Puckboong的一部分可以在大约两周的时间里从海峡收获一次。通过取走从水道中生长的白鹤，可以采取适当的管理措施来移除池塘水中的营养物质。此外，该杂交系统的主要材料证实在泰国很容易获得。

In this study, suppressing effect to harmful cyanobacteria, such as Microcystis by hybrid system which has combined channel planting hydrophyte for removal of nutrients and bioreactor fixing predacious microanimals was examined on experiment pond in Thailand. The fibroid carrier produced with waste plastic which had become unused in the locale was filled to the bioreactor, and Puckboong as eatable hydrophyte had been planted to the channel. The removal ability of toxic Microcystis was examined for a long term by this hybrid system, as a result the bioreactor achieved removal ratio more than 95%. Microanimal almost not detected on the experiment pond was gathered with high dense to the surface of carrier in bioreactor, and a lot of the microanimal community preying on Microcystis was observed. There is a possibility that microcystin remains in the bioreactor after the microanimal preys on Microcystis. As a result of the analysis that used high price performance liquid chromatography (HPLC), microcystin was not detected from outflow water of the bioreactor. This result suggests having the ability to produce enzymes by which either the microanimal or the bacteria in the bioreactor decomposes microcystin. The safety of hydrophyte as for food has improved because microcystin was completely decomposed though outflow water of the bioreactor enters the channel. The average removal ratio of nitrate-nitrogen and ammonium-nitrogen in the channel was 40% and 30% respectively. Moreover, a part of Puckboong that grew up was able to be harvested from the channel once about two weeks. Proper management to remove the nutrient from the pond water became possible by taking out Puckboong that grew up from the channel. In addition, the main material of this hybrid system confirmed obtaining was easy in Thailand.