In-Vivo microscopic observation of rabhit intrahcpatic haemodynamic change fir analysis of transcatheter arterial embolization therapy

为确定肝窦血流速度，在兔肝脏活体显微镜下，用摄像方法记录血细胞运动图像，以确定肝窦血流速度。然后用计算机软件对采集到的图像进行分析，最后计算出正弦血流速度。然而，根据兔的体温、心率、呼吸频率等身体条件的变化，血流速度在10分钟内会下降。根据这些结果，严格控制兔的身体状况是进一步分析正弦血流的必要手段。本研究旨在为利用兔等小动物的体内显微镜方法进行肝肿瘤经导管动脉栓塞术的基础研究。我们目前的结论是，使用更大的动物是重要的，以获得更好的体内显微镜观察数据，在没有动物状况变化引起的变异的情况下观察重要的肝脏血流。

To decide the hepatic sinusoidal flow velocity, at fires, the hematocyte moving images were recorded using video-capturing method under in-vivo microscopy of rabbit liver. And then the captured image analysis was done on computer software and finally estimated sinusoidal blood flow velocity was calculated.At first, average flow velocity of the sinusoids was about 30 pixel/flame. However, the flow velocity decreases within ten minutes according to the changes of rabbit body conditions such as body temperature and heart rates, respiratory rates. According to these results, strict control of rabbit body condition was necessary for further analysis of sinusoidal blood flow.This research aimed at the basic research for transcatheter arterial embolization of hepatic tumor using in-vivo microscopic method in small animal such as rabbit. And our current conclusion is to use bigger animal is important to get better data on in-vivo microscopic observation of vital liver blood flow without variability caused by animal condition change.