Degradation of Corrosion Resistance of Implant Metals in Biological effects with Adhesion Cells

为了保持人体内的生物相容性，金属植入物必须具有较高的耐腐蚀性。这项工作的目的是研究金属离子从植入材料释放到周围组织的机制。在这项研究中，作者研究了L929成纤维细胞和U937巨噬细胞形成的生物膜对纯镍板耐蚀性的生物效应(基于细胞的粘附性和(B)细胞的代谢活性)。纯镍并不是真正意义上的生物材料，但镍是一种易腐蚀的材料，可以用来研究不同实验条件下金属离子释放的变化，从而阐明其腐蚀机制。将L929成纤维细胞贴壁于金属表面，培养3d。然后，用icp-…测定培养基中溶解的镍离子的浓度。对于纯镍，镍离子浓度随着细胞密度的增加而增加。对于NiTi合金，Ni离子的溶出量比纯Ni的小，但Ni离子的浓度与晶胞密度有显著的相关性。当细胞蛋白质用10%甲醛溶液固定时，溶解的镍离子浓度与活细胞相比略有下降。结果表明，种植金属的耐腐蚀性与活的黏附细胞的存在密切相关。通过对电池金属腐蚀影响的研究，得出了以下结论。(1)金属种植体的体外腐蚀速度，细胞黏附的存在得到控制。锚定依赖性细胞(L929)促进金属离子的释放。(2)电池的代谢活性影响金属腐蚀。失去生物活性的细胞即使贴壁，也不会促进金属腐蚀。纤维连接蛋白等细胞黏附分子几乎不能促进金属离子的释放。较少

In order to maintain biocompatibility inside the human body, metallic implants must possess high corrosion resistance. The purpose of this work was to investigate the mechanism behind the release of metal ions from implant materials into the surrounding tissue. In this study, the authors examined the biological effects (based on (a) adhesion of cells and (b) cell metabolic activity) of biofilms formed by L929 fibroblastic cells and U937 macrophages on the corrosion resistance of a pure nickel plate. Pure Ni is not actually used as a biomaterial, but Ni, which corrodes easily, was used to investigate variations in metal ion release under different experimental conditions, and to clarify the corrosion mechanism.We studied on the affect of adhesive cells on the corrosion resistance of implant metal. L929 fibroblastic cells, which were adhered on the surface of the metals, were cultured for 3 days. Then, the concentration of Ni ion dissolved to the cultured medium was measured with the ICP … More analyzer.About pure Ni, the amount of Ni ion concentration was increased with the cell density. For NiTi alloy, the amount of dissolution of Ni ion was slighter than that of pure Ni, but the correlation of the concentration between Ni ion and the cell density was significant. On the other hand, when the protein of the cell was fixed with 10% formaldehyde solution, the dissolved Ni ion concentration was slightly compared with that of the living cells. As the results, it was suggested that the corrosion resistance of implant metal was closely depended on the existence of the living adhesive cells. The following conclusions have been reached in this study on the effect of metallic corrosion with cell. (1) The corrosion rate of metallic implant in vitro, the presence of the cell adhesion is controlled. An anchorage-dependent cell (L929) was promoted the release of metallic ions. (2) The metabolic activity of the cell influenced metallic corrosion. The cells that were lost the biological activities did not promote the metal corrosion even if the cells were adhered. Cell adhesion molecules (CAMs) such as fibronectin hardly promoted the release of metallic ions. Less