Improvement of biocompatibility of NiTi alloy by a cathode corrosion protection technique

阴极腐蚀防护技术提高NiTi合金生物相容性

• 批准号: 14580830
• 负责人: MORITA Masafumi
• 金额: $ 2.24万
• 依托单位: Saitama University, Faculty of Engineering (2003)Kitasato University (2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580830/
• 关键词: implant; Biomaterial; NiTI alloy; Biocompatibility; Shape Memory Alloy; Corrosion; Toxicity; マクロファージ; 生体材料; 生体親和性; 人工関節; Ni; 形状記憶合金; NiTi形状記憶合金; 腐食; 摩擦・磨耗; トライボロジー; 金属毒性

The NiTi alloy shows a unique, excellent mechanical properties like super-elasticity, a shape storage characteristic, and high wear resistance, etc. We designed the prevention of Ni ion release of the alloy by a cathode corrosion proof technique, and the improvement of the biocompatibility. We studied on the affect of adhesive cells on the corrosion resistance of implant metal. L929 fibroblastic cells, which were adhered on the surface of the metals, were cultured for 3 days. Then, the concentration of the Ni ion, which dissolved to the cultured medium was measured with the ICP analyzer. The toxicity test on Ni was carried out by using the L929 fibroblast and the U937 macrophage for a metallic powder and the corrosive liquid of metal Ni to achieve the above-mentioned purpose.About pure Ni, the amount of Ni ion concentration was increased proportionally to the cell density. For NiTi alloy, the amount of dissolution of the Ni ion was slighter than that of pure Ni, but the correlation of the concentration of Ni ion and the cell density was significant. On the other hand, when the protein of the cell was fixed with 10% formaline solution, the dissolved Ni ion concentration was a small amount compered with that of the living cells.According to the results, it was suggested that the corrosion resistance of implant metal was strongly depended on the existence of the living adhesive cells.

NiTi合金具有超弹性、形状存储特性、高耐磨性等独特的优良机械性能。我们设计了一种阴极防腐蚀技术来防止合金中Ni离子的释放，并改善其生物相容性。研究了粘附细胞对种植体金属耐腐蚀性能的影响。将粘附在金属表面上的L929成纤维细胞培养3天。然后，用ICP分析仪测量溶解到培养基中的Ni离子的浓度。为达到上述目的，用L929成纤维细胞和U937巨噬细胞对金属镍进行了毒性试验，结果表明，纯镍的镍离子浓度随细胞密度的增加而增加。对于NiTi合金，Ni离子的溶解量比纯Ni的溶解量小，但Ni离子浓度与晶胞密度之间存在显著的相关性。用10%福尔马林溶液固定细胞蛋白质时，溶解的Ni离子浓度比活细胞的要少，说明植入金属的耐蚀性很大程度上取决于活细胞的存在。

项目成果

水谷正義, 山田 藍, 小茂鳥潤, 片平和俊, 森田真史: "ELID研削を施した金属系生体材料の細胞毒性評価"第53期日本材料学会学術講演会講演論文集. 165-166 (2004)

Masayoshi Mizutani、Ai Yamada、Jun Komotori、Shun Kataheiwa、Masashi Morita：“ELID 研磨金属生物材料的细胞毒性评估”日本材料科学学会第 53 届年会论文集（2004 年）。

森田真史: "NiTi合金の生体適合性評価"日本機械学会誌. 7(印刷中). (2004)

Masashi Morita：“NiTi合金的生物相容性评估”日本机械工程学会杂志7（出版中）。

M.Morita, E.Kondo, T.Yamada, T.Sasada: "Electrochemical valuation of damage level to bearing surface of metal head joint prosthesis"MSRI. 9(1). 87-93 (2003)

M.Morita、E.Kondo、T.Yamada、T.Sasada：“金属头部关节假体支撑面损伤程度的电化学评估”MSRI。

T.Honma, Y.Kimura, M.morita: "Evaluation of Compatibility of Nickel-Titanium Shape Memory Alloy with Femur Surrounding Tissue"Proc.of the 14^<th> Jpn Mat.Res.Soc.. (in printing). (2003)

T.Honma、Y.Kimura、M.morita：“镍钛形状记忆合金与股骨周围组织的相容性评估”Proc.of the 14^<th>Jpn Mat.Res.Soc.（印刷中）。

橋本季子, 森田真史: "金属の耐食性に及ぼす接着性細胞の影響について"第53期日本材料学会学術講演会講演論文集. 167-168 (2004)

Kiko Hashimoto、Masashi Morita：“粘着细胞对金属耐腐蚀性的影响”日本材料科学学会第 53 届年会记录 167-168 (2004)。