Characterization of teleost hematopoietic stem cells

在硬骨鱼中，肾脏是主要的造血器官，在其中可以观察到所有的造血分化途径，包括红细胞生成、粒细胞生成和淋巴生成。然而，关于肾脏中的造血干细胞或造血祖细胞的信息很少。在本研究中，我们利用人参(Carassius Auratus Langsdorfii)和人参-金鱼杂交种的移植系统，证明了肾脏造血干细胞(HSCs)的存在。我们在18个月以上的受者体内发现了供者来源的三倍体血细胞，包括红细胞、血小板、粒细胞和淋巴细胞。这些结果表明，供体肾脏中存在长期的自我更新和多潜能的HSC。我们还在Hoechst染料排斥的基础上，利用侧群分析(SP)从人参和斑马鱼(Danio Rerio)的肾脏中分离出HSCs。通过体内长期再繁殖和多向分化潜能来评价人参SP细胞的肝星状细胞活性。

In teleosts, kidney is the major hematopoietic organ in which all lines of hematopoietic differentiation are observed including erythropoiesis, granulopoiesis, and lymphopoiesis. However, little information was available about the hematopoietic stem or progenitor cells in the kidney. In the present studies, we demonstrated the presence of hematopoietic stem cells (HSCs) in kidney hematopoietic tissues using the transplantation system of ginbuna (Carassius auratus langsdorfii) and ginbuna-gold fish hybrids.Kidney hematopoietic cells were obtained from ginbuna (triploid fish) and transplanted into ginbuna-gold fish hybrids (tetraploid fish). We found donor derived triploid blood cells including erythrocytes, thrombocytes, granulocytes and lymphocytes in the recipient for more than 18 months. These results indicate that long-term self-renewal and multipotent HSCs are present in the donor kidney.We also isolated HSCs from ginbuna and zebrafish (Danio rerio) kidney using side population (SP) analysis on the basis of Hoechst dye exclusion. The HSC activity of ginbuna SP cells were evaluated by their potential for long-term repopulation and multilineage differentiation in vivo.