Search on the genome scale of mRNAs that are translated under the abiotic stresses

植物在不同的非生物胁迫条件下，如高温、高盐、干旱、缺氧等条件下，都观察到了剧烈的翻译变化。翻译水平上对上述胁迫反应的共同特征是对翻译的整体抑制，以及存在一小部分对抑制不起作用的差异调控的mRNAs。然而，与转录水平相比，翻译水平上的调控或调控网络知之甚少。在本研究中，我们利用DNA微阵列技术，通过比较单个mRNAs在施加刺激前后的翻译状态，对高温(热胁迫)和高盐(盐胁迫)条件下拟南芥悬浮细胞培养中的翻译调控进行了全基因组分析。在此基础上，对两种应激条件下的翻译规则进行了比较。与以往关于单一非生物胁迫的报道相比，这种比较分析将有助于更好地理解植物对非生物胁迫反应的翻译控制的机制、调控网络或生物学意义。我们的结果表明，高温和高盐都能诱导细胞翻译抑制大多数基因的表达，但也有一部分细胞对这种抑制反应不敏感。值得注意的是，我们发现单个mRNA物种在翻译水平上的行为在植物对这两种胁迫的反应中都是相似的。

Drastic translational changes have been observed in plants under various abiotic stress conditions such as elevated temperature, high salinity, drought, and O2 deprivation. Common features of responses at the translational level to above stresses are global repression of translation and the existence of a fraction of differentially regulated mRNAs that are refractory to the repression. Less is known, however, about the regulation or regulatory networks at the translational levels compared to the transcriptional levels.In this study, we carried out genome-wide analysis of translational regulation in Arabidopsis (Arabidopsis thaliana) suspension cell culture subjected to elevated temperature (heat stress) and high salinity (salt stress) conditions using DNA microarray by comparing the translation state of individual mRNAs before and after the application of a stimulus. On that basis, translational regulations under the both stress conditions were compared. It was expected that this comparative analysis would allow gaining a better understanding of the mechanism, the regulatory network, or biological significance of translational control in plants response to abiotic stress than previous reports focused on a single abiotic stress. Our results showed that both elevated temperature and high salinity elicit the cells to translation repression of most mRNA species, while some were defied to or sensitive to the repression. Notably, we found that the behavior of individual mRNA species at the translational levels were similar between in plants response to both stresses.