Association with occult hepatitis C virus in the patients with non-alcoholic steatohepatitis

为定性定量检测非酒精性脂肪性肝炎（NASH）患者中隐匿性丙型肝炎病毒（HCV），对血清HCV-RNA阳性的NASH患者进行HCV-RNA实时荧光定量PCR检测（阳性对照，n=6）、血清HCV-RNA阴性的NASH患者（n=47）和患有其他疾病的患者，例如良性肿瘤、转移性肝肿瘤，血清HCV-RNA阴性的药物性肝损伤和胆管瘤及NASH（阴性对照，n=14）。对10 mg肝组织进行真实的PCR检测是必要的。所有慢性丙型肝炎患者的真实的时间PCR结果均为阳性（4.7±6.5x10^5拷贝/检测，平均值±SD，范围：1.1-8.5x10^5拷贝/检测。真实的时间PCR检测10 mg肝组织中病毒载量与确诊患者血清中病毒载量显著相关（p<0.05）。而NASH及其他血清HCV-RNA阴性的疾病患者均未检出HCV-RNA。此外，我们还应用实时荧光定量PCR技术检测了4例肝细胞癌和非酒精性脂肪性肝炎患者的肝组织标本中的HCV-RNA。与NASH患者一样，所有患者的真实的时间PCR结果均为阴性。这一结果表明隐匿性HCV与NASH的二次打击理论无关。

In order to qualitatively and quantitatively detect the occult hepatitis C virus (HCV) in the patients with non-alcoholic steatohepatitis (NASH) , we underwent real-time PCR for HCV-RNA in the patients whose serum HCV RNA were positive (positive control, n=6) , with NASH whose serum HCV-RNA were negative (n=47) and the patients with other diseases example for benign tumor, metastatic liver tumor, drug-induced liver injury and cholangioma whose serum HCV-RNA were negative as well as NASH (negative control, n=14). lassay of real time PCR was necessary for 10 mg of liver tissue. The results in real time PCR were positive in all of the patients with chronic hepatitis C (4.7±6.5x10^5 copies/assay, mean±SD, range: 1.1-8.5x10^5 copies/assay. The viral load in 10mg of liver tissue estimated by Real time PCR significantly related to serum viral load in identified patient with hepatitis C (p<0.05) . However, HCV-RNA was not detected in the patients with NASH and other diseases whose serum HCV-RNA were negative. In addition, we demonstrated the real-time PCR for HCV-RNA using by resected liver specimen in the four patients with burned out NASH and hepatocellular carcinoma. As well as the patients with NASH, the results in real time PCR were negative in all of the patients. This results suggested that occult HCV did not associated with second hit theory for NASH.