Analysis of cerebral microcirculatory regulation in relation to anesthetics and gene therapy using the live-cell imaging

我们采用计算机辅助显微镜技术对大鼠脑片微血管功能进行了研究。电刺激引起脑实质小动脉收缩，而20-HETE的抑制剂消除了这种反应。兴奋性氨基酸之一的NMDA引起这些小动脉的扩张，对应于平滑肌细胞中除了一氧化氮之外的超氧化物的细胞内产生。在临床相关浓度范围内的丙泊酚可降低这些血管反应。

We employed the computer-assisted microscopy to evaluate microvascular function in the rat brain slices. Electrical stimulation induced constriction of the cerebral parenchymal arteriole, whereas an inhibitor of 20-HETE abolished this response. NMDA, which is one of excitatory amino acids, produced dilation of these arterioles, corresponding with intracellular production of superoxide in addition to nitric oxide in the smooth muscle cells. Propofol within clinically relevant concentrations reduced these vascular responses.