Clarifying the mechanism on systemic acquired resistance in Japanese birch plants infected with a canker-rot fungus, Inonotus obliquus

以日本白桦8号试管苗为材料，检测并鉴定了白桦溃疡病菌Inonotus numerus IO-U1菌株侵染初期的侵染特异性蛋白。结果发现，在感染真菌的8号试管苗中，氧化爆发和系统获得抗性作为保护性反应发生。此外，还对感染IO-U1菌株的8号和东北两种试管苗进行了解剖学和组织化学观察。因此，这表明酚类化合物及其聚合物的沉积和木栓化的坏死性菌丝体的形成抑制了真菌菌丝在两种感染真菌的试管苗中的生长。并对8号试管苗的过氧化物酶同工酶进行了分析。因此，这表明，过氧化物酶同工酶的感染苗专门为酚类化合物的聚合。此外，它是通过使用蛋白质组学分析研究是否发生在8号植株的SAR诱导化合物，水杨酸人工管理的系统获得性抗性（SAR）。结果表明，对8号幼苗施用水杨酸诱导了幼苗中的SAR。此外，有人认为，发生的抗性机制之间的情况下，真菌感染和水杨酸的管理到试管苗是不同的。

The fungal infection specific proteins were detected and identified for the Japanese birch plantlets No.8 infected with a canker-rot fungus of birch trees, Inonotus obliquus IO-U1 strain at the early stage of their fungal infection. As a result, it was found that oxidative burst and systemic acquired resistance occur as the protective reactions in the plantlets No.8 infected with the fungus. In addition, anatomical and histochemical observations were performed for the two kinds of plantlets, No.8 and Tohoku, infected with IO-U1 strain. Consequently, it was suggested that deposition of phenolic compounds and their polymerized substance, and the formation of suberized necrophylactic periderm inhibit the growth of the fungal hyphae in the two kinds of plantlets infected with the fungus. Furthermore, isozyme analysis of the peroxidases produced in the plantlets No.8 was carried out. In consequence, it was indicated that the peroxidase isozymes of the infected plantlets are specialized for polymerization of phenolic compounds. Moreover, it was investigated by using proteome analysis whether systemic acquired resistance (SAR) occurs in the plantlets No.8 to which the SAR-inducing compound, salicylic acid was artificially administered. As a result, it was indicated that administration of salicylic acid to the plantlets No.8 induces SAR in the plantlets. In addition, it was suggested that the occurring resistance mechanisms are different between the case of the fungal infection and that of administration of salicylic acid to the plantlets.