The screening of the new functional compounds for the prevention of cardiovascular events.

血管平滑肌（VSM）的异常收缩（血管痉挛）是引起心绞痛和心肌梗死的重要现象之一。我们以前发现，鞘氨醇磷酸胆碱（SPC）通过激活Src家族酪氨酸激酶成员Fyn作为异常通路的上游介质。Fyn定位于富含胆固醇的膜微区，脂筏，表明膜脂筏在异常收缩中的重要作用。因此，我们研究了脂筏与SPC诱导的VSM Ca^<2+>增敏的关系。此外，我们还筛选了能够抑制SPC诱导的Ca^2+-敏化的新化合物。一些新发现的化合物有望成为治疗血管痉挛的新型功能性食品。

Abnormal vascular smooth muscle (VSM)contraction (vasospasm) is one of the crucial phenomena which cause cardiac angina and myocardial infarction. We previously found that sphingosylphosphorylcholine (SPC) as an upstream mediator for the abnormal pathway through the activation of Fyn, a member of Src family tyrosine kinase. The localization of Fyn in cholesterol-enriched membrane microdomain, lipid rafts, suggest the essential roles of membrane lipid rafts in abnormal contraction. Therefore, we investigated the relationship between lipid rafts and SPC-induced Ca^<2+>-sensitization of VSM. In addition we screened novel compounds which could inhibit SPC-induced Ca^<2+>-sensitization. Some newly found compounds would be the candidates for novel functional food for vasospasm.