The elucidation of molecular mechanism of vasospasm and the development of the prophylactic treatment with innovative natural ingredient.

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Abnormal vascular smooth muscle (VSM)contraction (vasospasm) is one of the crucial phenomena which cause cardiac angina and myocardial infarction. We previously found that sphingosylphosphorylcholine (SPC) as an upstream mediator for the abnormal pathway through the activation of Fyn, a member of Src family tyrosine kinase. The localization of Fyn in cholesterol-enriched membrane microdomain, lipid rafts, suggest the essential roles of membrane lipid rafts in abnormal contraction. Therefore, we investigated the relationship between lipid rafts and SPC-induced Ca2+-sensitization of VSM. In addition, high performance liquid chromatography (HPLC) and mass spectrometry (MS) revealed prophylactic compounds that can markedly inhibit the Ca2+-sensitization, without affecting physiological Ca2+-dependent contraction. These results suggest that innovative natural ingredient may contain novel protective components active against abnormal VSM contraction.