Developmental change, effects of stress and GIRK channel inhibitor on GIRK channel function.

为了研究早期生活应激对发育中的大鼠大脑中GIRK通道功能变化的影响，从出生后第2天至第14天，每天1小时或从出生后第2天至第9天，每天3 ~ 4小时给予母剥夺应激。结果，5-HT诱导的海马神经元GIRK电流（I_<5-HT>）升高。海马区GIRK2表达水平下降，而前额叶皮层和中缝核区表达水平未见下降。行为实验焦虑水平指数降低。这表明MD应激影响海马中GIRK通道的功能。

To investigate the effects of early life stress on the functional change of GIRK channel in developing brain, rat pops were given maternal deprivation (MD) stress for 1h a day from postnatal day (PND) 2 to 14 or for 3 to 4h a day from PND2 to 9. As a result, the 5-HT-induced GIRK current (I_<5-HT>) was increased in hippocampal neurons. GIRK2 expression level was decreased in the hippocampus but not in prefrontal cortex and raphe nucleus. Anxiety level index in behavioral experiment was decreased. These suggest that MD stress affects the function of GIRK channel in the hippocampus.