In vivo real time imaging of esophageal cancer with CdSe nanoparticle

我们开发了一种超稳定的抗Her2抗体偶联的CdSe纳米颗粒，作为一种新型的功能荧光探针用于体内成像。静脉注射该纳米粒子后，我们成功地从体外检测到荷瘤裸鼠体内人食道癌的荧光信号和图像。此外，我们还在临床应用的基础上开发了新的内窥镜荧光手术系统。利用该荧光检测系统，以CdSe为纳米示踪剂，成功地对猪模型胃肠道淋巴网络和前哨淋巴结活检进行了实时成像。

We developed ultra-stable nanoparticles of CdSe conjugated with anti-Her2 antibody as a novel functional fluorescent probe for in vivo imaging. After intravenous injection of this nanoparticle, we were able to detect the fluorescent signals and image of human esophageal cancer in tumor-bearing nude mouse from outside the body successfully. Furthermore, we developed new surgical system for endoscopic fluorescent surgery based on clinical application. Using this fluorescent detection system and CdSe as a nano-tracer, we succeeded in real time imaging of lymph networks and sentinel lymph node biopsy of gastrointestinal tract in pig model.