Establishment of patho-physiological diagnosis and rational treatment protocol for apical periodontitis using qualitative and quantitative analysis of intracanal microflora.

观察根尖周炎患者在根管治疗过程中管内菌群的变化。采集牙本质样本前获得知情同意。对样本进行厌氧培养并进行分子遗传学分析，结果表明：根据患者的治疗史和口腔可及性等临床条件，患者的微生物组成存在显著差异；检出最多的菌种为假弧菌、奥氏菌、丙酸菌、乳杆菌；无论细菌种类或细菌负荷如何，常规治疗方案通常能够控制肛门内感染。

Changes of intracanal microflora during the course of root canal therapy were investigated in patients with apical peridontitis. Informed consent was obtained prior to dentin sample collection. Anaerobic culture of the samples followed by molecular genetic analysis revealed that : a marked variation of the microbial composition was found among the cases according to their clinical conditions such as history of treatment and accessibility to oral cavity ; Pseudoramibacter, Olsenella, Propionibacterium, Lactobacillus were the frequently-detected species; the conventional treatment protocol was usually capable of maneuvering intracanal infection regardless of bacterial species or bacterial loads.