Whole genome screening of plant membrane transporter for inorganic elements

植物细胞包含了宇宙的所有元素。输入/输出无机元素的转运体尚未得到很好的识别。在拟南芥基因组中，约有1,400个转运蛋白相关基因被计算机模拟发现，其中的功能几乎是未知的。为了克服从可能的候选者中进行全基因组筛选，我们构建了由转运蛋白基因组成的表达文库。我们克隆了1,380个基因（98%的候选基因）。通过使用文库进行表达筛选已被证明是鉴定新型转运蛋白的有力工具。本研究筛选出了5个与宿主生物对重金属元素（Mn、Ni、Zn、Cd）的耐受性相关的候选基因。我们还开发了可以分析生物体中元素水平的研究工具。

Plant cells contain all elements of the universe. Transporters that import/export inorganic elements have been poorly identified yet. In Arabidopsis genome, about 1,400 transporter-related genes were fond in silico, of which function is almost unknown. To overcome whole genome screening from possible candidates, we constructed an expression library consisted of the transporter gene(s). We cloned 1,380 genes (98% of candidates). Expression screening by use of the library has been shown to be powerful tool for identification of novel transporters. We screened five candidate genes that posed tolerance of heavy metal elements (Mn, Ni, Zn, Cd) to host organisms. We also developed research tools that can analyze level(s) of element(s) in living organisms.