Precise synthesis of glycoproteins having human oligosaccharides by use of chemical and expression methodologies.

合成促红细胞生成素类似物和共刺激糖蛋白。在此基础上，还合成了IgG-Fc区糖基化多肽链。为了合成糖蛋白，采用化学方法制备糖肽-硫酯，采用大肠杆菌表达法制备多肽链。将两肽偶联制备完整的糖基化多肽，然后进行折叠，得到折叠后的糖蛋白。

Erythropoietin analogue and co-stimulate glycoprotein were synthesized. In addition to these results, glycosylated polypeptide chain of IgG-Fc region was also synthesized. In order to synthesize glycoproteins, glycopeptides-thioesters were prepared by chemical method and polypeptide chain was prepared from E. Coli expression method. Coupling both peptides to prepare whole glycosylated polypeptide and subsequent folding process afforded the folded glycoproteins.