Study on the common ancestors of Archaea, Bacteria and Commonote based on the resurrected enzymes.
Study on the common ancestors of Archaea, Bacteria and Commonote based on the resurrected enzymes.
批准号:
20370094
负责人:
YAMAGISHI Akihiko
金额:
$13.15万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2010
中文摘要
恢复了翻译系统(GlyRS)、代谢系统(IPMDH和NDK)和复制系统(Gyrase)的祖先酶,构建了它们的基因,并在大肠杆菌中进行了表达。翻译和复制系统的纯化酶比极端嗜热菌的相应部分表现出更高的热稳定性。分析了先前构建的祖先NDH的几个突变体。结果表明,细菌、古生菌和共生菌的祖先酶具有极高的热稳定性(>;100℃)。
英文摘要
Ancestral enzymes of translation system (GlyRS), metabolic system (IPMDH and NDK) and replication system (gyrase) were resurrected, their genes were constructed and expressed in E. coli. The purified enzymes of translation and replication systems showed higher thermal stability than counter parts of an extreme thermophile. Several mutants of previously constructed ancestral NDH were analyzed. The results showed that ancestral enzymes of Bacteria, Archaea and Commonote are extremely (>100℃) thermostable.
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Thermal stability and catalytic properties of resurrected ancestral types of nucleoside diphosphate Kinase
复活祖先类型核苷二磷酸激酶的热稳定性和催化特性
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[赤沼哲史, 横堀伸一, 小林愛美, 山岸明彦]
通讯作者:
山岸明彦
復元した祖先型DNAシャイレースの熱安定性
恢复祖先 DNA 系带的热稳定性
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[横井珠希, 岩見祥子, 金城健太, 渡邊英哲, 横堀伸一, 赤沼哲史, 山岸明彦]
通讯作者:
山岸明彦
第1章 生命の起源と初期進化、海洋生命系のダイナミクス・シリーズ第1巻「海洋の生命史」
第一章:生命的起源和早期进化,海洋生命系统的动力学系列第一卷:海洋生命的历史
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[山岸明彦]
通讯作者:
山岸明彦
酵素の祖先型改変耐熱化
祖先对酶进行改造,使其更加热稳定
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[Yoshihiro Shimizu, Bei-Wen Ying, Junya Ichinose, Yoichiro Ito, Tetsuya Yomo, 覚張隆史・山崎京美・米田穣, 山岸明彦]
通讯作者:
山岸明彦
祖先型アミノ酸配列から成るDNAシャイレースBサブユニットの解析
由祖先氨基酸序列组成的 DNA shylace B 亚基的分析
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[岩見祥子, 横井珠希, 金城健太, 渡辺英哲, 横堀伸一, 赤沼哲史, 山岸明彦]
通讯作者:
山岸明彦
共 49 条
Origin of phagocytosis in eukaryotic cells
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批准号:24657165
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.58万
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财政年份:2012
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负责人:YAMAGISHI Akihiko
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依托单位:
Analysis of the proteins possessed by the last common ancestor and the organisms present before it
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批准号:23370097
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$6.49万
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财政年份:2011
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负责人:YAMAGISHI Akihiko
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依托单位:
Searches for clay minerals with asymmetric structures and their identification
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批准号:19340159
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.15万
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财政年份:2007
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负责人:YAMAGISHI Akihiko
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依托单位:
Syntheses of Biominerals under the Template of Artificial Miomembranes
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批准号:15340184
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.98万
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财政年份:2003
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负责人:YAMAGISHI Akihiko
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依托单位:
Development of Energy Conversion Systems by Use of Inorganic/Organic Hybrid LB Films
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批准号:14050027
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项目类别:Grant-in-Aid for Scientific Research on Priority Areas
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资助金额:$20.54万
-
财政年份:2001
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负责人:YAMAGISHI Akihiko
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依托单位:
Application of Clay-Metal Complex Hybrid Films to Non-linear Optical Materials
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批准号:10044046
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项目类别:Grant-in-Aid for Scientific Research (B).
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资助金额:$2.69万
-
财政年份:1998
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负责人:YAMAGISHI Akihiko
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依托单位:
Study of the cytoskeleton and lipid biosynthetic system of an archaeon Thermoplasma
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批准号:09839033
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.73万
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财政年份:1997
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负责人:YAMAGISHI Akihiko
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依托单位:
Preparation of Clay Monolayr and its Application of Electrode Modifier for Asymmetric Syntheses
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批准号:07404039
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$13.25万
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财政年份:1995
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负责人:YAMAGISHI Akihiko
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依托单位:
Development fo a column material for chromatographic resolution, using an ion-exchanged adduct of clay-alubumin.
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批准号:06554030
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项目类别:Grant-in-Aid for Developmental Scientific Research (B)
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资助金额:$4.86万
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财政年份:1994
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负责人:YAMAGISHI Akihiko
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依托单位:
Asymmetric Syntheses by Use of Intercalation of Multi-nuclear Chiral Metal Complexes into a Clay
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批准号:05453048
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$3.39万
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财政年份:1993
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负责人:YAMAGISHI Akihiko
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依托单位:
Development of a new method for choromatographic resolution by use of a clay
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批准号:03554024
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项目类别:Grant-in-Aid for Developmental Scientific Research (B)
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资助金额:$3.33万
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财政年份:1991
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负责人:YAMAGISHI Akihiko
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依托单位:
Studies on Stereoselectivities on a Clay Surface by STM Method
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批准号:01470006
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$4.16万
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财政年份:1989
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负责人:YAMAGISHI Akihiko
-
依托单位:
海外基金