Studies on screening for bioactive compounds targeting gene repair, recombination and splicing systems.

肿瘤细胞对抗肿瘤药物产生耐药性是肿瘤化疗的重要问题之一，基因修复机制参与了这一过程。因此，基因修复系统被认为是开发抗肿瘤药物的有前途的分子靶点。除了修复系统外，转录和修饰如剪接系统也被证明是癌症化疗的一个有吸引力的靶点。在这项研究中，我们成功地构建了良好的检测系统来监测基因修复和剪接活性，并成功地从天然产物中发现了这些活性的抑制剂，包括微生物次级代谢物，作为广泛药物筛选的结果。

One of the significant problems about the cancer chemotherapy is the acquisition of resistance to antitumor agents against cancer cells, in which the gene repair mechanisms takes part in. Thus, gene repair system is considered to be the promising molecular target for the development of anti-tumor agents. In addition to repair system, transcription and modification such as splicing system is also shown to be attractive target for cancer chemotherapy.In this research, we succeeded to construct the good assay systems to monitor gene repair and splicing activities, and also succeeded to discover inhibitors against these activities from natural products including microbial secondary metabolites as the results of wide drug screenings.