Regulation assay of light dependent transcription using a cell-free system derived from Arabidopsis

从快速生长的拟南芥培养细胞MM2d系中建立了两种无细胞转录检测系统。培养的细胞在黑暗条件下呈黄化，在光照条件下呈绿色。利用这两种不同颜色的细胞，建立了体外转录系统。然后，利用这两种体外转录系统，我们成功地分析了拟南芥核酮糖-1,5-二磷酸羧化酶/加氧酶（红细胞）基因的光依赖性转录调控。

Two Cell-free transcription assay systems were established from rapidly growing Arabidopsis cultured cell MM2d line. The cultured cells were etiolated when the cells were growing under dark condition and were greening when the cells were growing under light condition. Using these two different colored cells, in vitro transcription systems were established. And then, using the two in vitro transcription systems, we have successfully assayed light dependent transcription regulation using Arabidopsis ribulose-1,5-bis-phosphate carboxylase/oxygenase (rbcS) genes.