Interaction between Potyvirus viral protein linked to the genome and host cell protein

为了研究TuMV VPG与eIF4E结合的特异性，我们用SPR方法研究了人eIF4E、线虫IFE-3和IFE-5与拟南芥eIFso4E相互作用的动力学参数。结果表明，在帽子结合的eIF4E同源基因中，VPG与构成帽子结合口袋入口一侧的C-末端区域结合，而在无帽状态下，VPG与广泛打开的帽子结合口袋及其周围区域结合。

To investigate the binding specificity of TuMV VPg with eIF4E, we evaluated the kinetic parameters for the interactions of human eIF4E, C. elegans IFE-3 and IFE-5 and Arabidopsis eIFiso4E, by SPR. The results suggested that in cap-bound eIF4E orthologues, the VPg binds to the C-terminal region, which constitutes one side of the entrance to the cap-binding pocket, whereas in the cap-free state, VPg binds to the widely opened cap-binding pocket and its surrounding region.