Development and application of determination methods of salivary hormones for noninvasive diagnosis of endocrine disorders

唾液在临床检查中作为一种非侵入性的检查工具，最近引起了人们的注意。本研究建立了高灵敏、高专属性的高效液相色谱-电喷雾电离-串联质谱法测定唾液中的低分子激素及其相关化合物。对于一些化合物，开发了新的衍生化程序来增强其检测响应。该方法可用于先天性肾上腺增生症、维生素D缺乏症、晚发型性腺功能减退症和Graves病等疾病的诊断和治疗。

Saliva has recently been attracting attention as a noninvasive tool in clinical examinations. In this study, highly sensitive and specific liquid chromatography?electrospray ionization-tandem mass spectrometry methods were developed for the determination of low-molecular weight hormones and related compounds in saliva. For some compounds, novel derivatization procedures were developed to enhance their detection responses. The developed methods were useful in the diagnosis and treatment for several diseases and deficiencies, such as congenital adrenal hyperplasia, vitamin D deficiency, late-onset hypogonadism and Graves disease.