Scientific research of evidence-based prevention of illegal drug of abuse

“指定物质”的结构分为三种类型，即苯乙胺、色胺和胡椒胺。建立了高效液相色谱-多通道电化学检测同时测定指定物质的方法。提出的在线氧化方法是首次报道了多种指定物质的同时测定。同时建立了超高效液相色谱-荧光检测-电喷雾飞行时间质谱仪同时测定16种指定物质的方法。然而，FL检测中几种指定物质的测定受到内源性物质的干扰。然而，实际样品中的测定是通过UPLC分离和TOF-MS检测相结合的方式成功进行的。该方法已用于人体血浆、尿液和实际产品的分析。此外，利用手性试剂R(-)-DBD-PyNCS间接手性衍生化方法实现了结构中含有不对称碳的苯乙胺的对映体拆分。用反相高效液相色谱法对12种苯乙胺类化合物进行了高效分离和灵敏检测。该方法已成功应用于大鼠血浆和实际产品的分析。

The structures of "designated substances" (Shitei-Yakubutsu) are divided into three types, i.e., phenethylamines, tryptamines and piperadines. Simultaneous determination method using HPLC with multi-channel electrochemical detection was developed for the designated substances. The proposed method utilizing online oxidation was the first report on the simultaneous determination of various designated substances. The simultaneous determination based on UPLC with FL detection and ESI-TOF-MS was also developed for 16 designated substances. However, the determination of several designated substances by FL detection was interfered with endogenous substances. However, the determination in real samples was successfully carried out by a combination of UPLC separation and TOF-MS detection. This method was applied to the analyses of human plasma, urine and real products. Furthermore, the enantioseparation of phenethylamines possessing an assymetric carbon in the structure was achieved with indirect chiral derivatization method using a chiral reagent, R(-)-DBD-PyNCS. Twelve kinds of phenethylamine derivatives were efficiently separated by reversed-phase HPLC and sensitively detected. The proposed method was successfully applied to the analyses of rat plasma and real products.