Molecular mechanism of abnormal pH-homeostasis in the lumen of acidic organele from DjA2 knock out mice

DjA2基因敲除小鼠在出生后表现出致命的呼吸衰竭。这是由于肺泡扩张不充分和基因敲除小鼠的2型肺细胞溶酶体酸性细胞器内腔酸性异常所致。为了阐明维持酸性细胞器内稳态的分子机制，我分析了pnumocytes的生理方法，使用比率法，DjA2的分子相互作用研究，使用Biacore，和肺组织的蛋白质组学方法。

The DjA2 knockout mice showed lethal respiratory failure after birth. This was caused by insufficient alveolar expansion and the type 2 pneumocytes from the knockout mice had abnormal acidity in the lumen of lysosomal acidic organelle. To elucidate the molecular mechanism of maintaining homeostasis in the acidic organelle, I analyzed the pnumocytes by a physiological approach using ratiometry, the DjA2 by a molecular interaction study using Biacore, and the lung tissue by a proteomics approach.