Role of connexin-43 in protective signaling incardiomyocyte

本研究旨在阐明连接蛋白43 （Cx43）在心肌细胞中主要的细胞保护信号传导PI3K-Akt-GSK-3β信号传导中的作用。我们发现，肌膜中的Cx43作为G蛋白β亚基的辅助因子，有助于I_B类P13K的激活。Cx43的这一作用特异于I_B类P13K的调控，而不是I_A类PI3K的激活所必需的。线粒体中的Cx43是激活线粒体atp敏感的K^+通道的氧化还原信号所必需的，而在g蛋白偶联受体的PI3K-Akt-GSK-3β信号传导中则是必不可少的。

This study aimed to clarify role of connexin-43 (Cx43) in a major cytoprotective signaling, PI3K-Akt-GSK-3β signaling in cardiomyocytes. We found that Cx43 in the sarcolemma contributes to activation of class I_B P13K as a co-factor of β subunit of G proteins. This role of Cx43 is specific to regulation of class I_B P13K and is not required for activation of class I_A PI3K. Cx43 in mitochondria, being necessary for redox signaling by activated mitochondrial ATP-sensitive K^+ channel, was found to be dispensable in PI3K-Akt-GSK-3β signaling by G-protein coupled receptors.