Development of HSCCC/MS for the efficient purification of natural actives.

本研究旨在开发用于高速逆流色谱(HSCCC)的有效纯化方法，该方法不存在不可逆吸附，具有与柱层析相当的高分辨率。HSCCC已广泛应用于天然、医药和生化产品的制备分离和提纯。我们的研究报告了HSCCC系统用于电喷雾电离在线监测庆大霉素和多肽。这项技术将用于生物活性化合物的提纯，用于连续的质谱学数据采集，以及目标引导的小分子/大分子的分级。本文建立了高效毛细管气相色谱-质谱联用技术，用于天然提取物中生物活性物质的纯化。

This study aims to develop useful purification utilized with mass spectrometric monitoring for high-speed counter-current chromatography (HSCCC), which is free of irreversible adsorption and offers high resolution comparable to column chromatography. HSCCC has been widely used in preparative separation and purification of natural, medical and biochemical products. Our study was reported that HSCCC system was efficiently applied to separation of the gentamicin and polypeptide using on-line mass spectrometric monitoring with electrospray ionization. This technique would be useful purification of bioactive compounds for continuous mass spectrometric data acquisition, and 'target'-guided fractionation of small/large molecular. In this report, the HSCCC coupled with mass spectrometry technique was developed for the purification of bioactive compounds in natural extracts.