The role of Chemokine receptor CXCR3 in colon cancer metastasis

SW480细胞系来源于原发性杜克B结肠癌，而SW620细胞系来源于同一患者的淋巴结转移。CXCR3和CXCR4在SW620中的表达量均高于SW480。在两种细胞系中，配体与CXCR3或CXCR4相互作用可诱导细胞迁移、侵袭和增殖。重要的是，当CXCR3被其配体预先激活时，cxcr4诱导的迁移和侵袭被促进。这些结果提示，CXCR3可能通过与CXCR4协同作用，在结肠癌转移中发挥作用。我们利用miRNA建立了三个稳定的细胞系；CXCR3敲除、CXCR4敲除和CXCR3/CXCR4双敲除细胞系。我们用裸鼠直肠移植模型比较了这三种细胞系与对照细胞的转移潜能。

The SW480 cell line was derived from a primary Duke B colon cancer, whereas the SW620 cell line was derived from a lymph node metastasis of the same patient. Both CXCR3 and CXCR4 were much higher expressed in the SW620 than in the SW480. Cell migration, invasion and proliferation were induced by interaction of ligands with CXCR3 or CXCR4 in both cell lines. Importantly, CXCR4-induced migration and invasion were promoted when CXCR3 was pre-activated with its ligands in advance. These results suggest that CXCR3 can play a role in colon cancer metastasis by cooperating with CXCR4 synergistically. We established three stable cell lines using miRNA; CXCR3 knockout, CXCR4 knockout and CXCR3/CXCR4 double knockout cell lines. We have compared the metastatic potential of the three cell lines with the control cells by rectal transplantation model in nude mice.