Genome wide localization analysis of BphT1 binding region on Rhodococcus jostii RHA1.

Jostii红球菌RHA1生长在广泛的芳香族化合物上，并能有效降解多氯联苯（PCBs）。双组分信号转导系统BphT1T2是R. jostii RHA1.在这项研究中，我们进行了全球染色质免疫沉淀芯片（ChIP芯片）分析，以确定新的BphT1结合区域在R。jostii RHA1.结果发现9个ORF是BphS1T1的直接靶基因，包括IclR型转录调控因子、可能的内切酶和一些假设的蛋白质。这一结果表明BphS1T1是联苯降解的主调节因子，并直接激活转录调节因子和核糖核酸内切酶来调节RHA1中许多ORF的表达水平。

Rhodococcus jostii RHA1 was grown on a broad range of aromatic compounds and vigorously degrades polychlorinated biphenyls (PCBs). Two-component signal transduction system, BphT1T2, is necessary for activation of biphenyl degradation genes in R. jostii RHA1. In this study, we performed global chromatin immunopercipitation-on-chip (ChIP-chip) analysis to identify novel BphT1 binding region in R. jostii RHA1. As a result, we identified 9 ORFs as direct target of BphS1T1, which including IclR type transcriptional regulator, possible endonuclease, and some hypothetical proteins. This result indicated that BphS1T1 is a master regulator of biphenyl degradation, and directly activated to transcriptional regulator and endonibonuclease to regulate expression level of many ORFs in RHA1.