The functional analysis of exposure to a MRI-type high-strength static magnetic field stimulates megakaryocytic/erythroid hematopoiesis in CD34+ cells from human placental and umbilical cord blood

暴露在高强度静磁场(SMF)中后的生物反应最近从可能的健康益处和潜在的不利影响的角度被广泛讨论。为了阐明这一问题，我们在高强度SMF的条件下体外暴露了人胎盘和脐带血中的CD34^+细胞。高强度SMF曝光系统由磁场发生器、无氦超导磁体和内置CO_2孵化器组成。新鲜制备的CD34细胞暴露于磁场梯度最强的5特斯拉(T)SMF(41.7T/m)和无磁场梯度的10T SMF作用4或16h后，收获的CD34细胞经10T SMF作用16h后，红系和巨核系祖细胞集落形成显著增加，分别是对照组的1.72倍和1.77倍。此外，暴露于SMF后，早期造血相关基因和细胞周期相关基因显著上调。这些结果表明，10T SMF暴露可能改变基因表达，导致多能造血干细胞向巨核/红系祖细胞分化的特异性增强和/或双能MEP的增殖。

The biological response after exposure to a high-strength static magnetic field (SMF) has recently been widely discussed from the perspective of possible health benefits as well as potential adverse effects. To clarify this issue, CD34^+ cells from human placental and umbilical cord blood were exposed under conditions of high-strength SMF in vitro. The high-strength SMF exposure system was comprised of a magnetic field generator with a helium-free superconducting magnet with built-in CO_2 incubator. Freshly prepared CD34 cells were exposed to a 5 tesla (T) SMF with the strongest magnetic field gradient (41.7T/m) or a 10 T SMF without magnetic field gradient for 4 or 16 h. In the harvested cells after exposure to 10 T SMF for 16 h, a significant increase of hematopoietic progenitors in the total burst-forming unit erythroid- and megakaryocytic progenitor cells-derived colony formation was observed, thus producing 1.72- and 1.77-fold higher than the control, respectively. Furthermore, early hematopoiesis-related and cell cycle-related genes were found to be significantly up-regulated by exposure to SMF. These results suggest that the 10 T SMF exposure may change gene expressions and result in the specific enhancement of megakaryocytic/erythroid progenitor (MEP) differentiation from pluripotent hematopoietic stem cells and/or the proliferation of bipotent MEP.