Homing of circulating osteogenic progenitors to sites of fracture repair under the low-intensity pulsed ultrasound treatment

通过手术连接一只转基因GFP小鼠和一只野生型伴侣来创建联体模型。在建立共享循环后，在野生型伴侣的对侧股骨干中创建了横向骨折伴骨膜剥离。骨折部位每天暴露于低强度脉冲超声（LIPUS）治疗。在骨形成部位鉴定了通过体循环进入截骨部位的GFP+细胞。结果表明，LIPUS诱导循环成骨祖细胞归巢到截骨部位，可能有助于新骨形成。

A parabiotic model was created by surgically conjoining one transgenic GFP mouse and one wild-type partner. After shared circulation was established, a transverse fracture with periosteal stripping was created in the contralateral diaphysial femur of the wild-type partner. The fracture site was treated with daily exposure of low-intensity pulsed ultrasound (LIPUS). GFP+ cells that gained access to the osteotomy site via the systemic circulation were identified in the bone-forming site. The findings suggested that LIPUS induced homing of circulating osteogenic progenitors to the osteotomy site for possible contribution to new bone formation.