Construction of a screening method for target proteins of protein arginine methyltransferases

本研究的目的是建立一个基于mRNA展示技术的PRMT特异性底物的体外筛选系统，称为体外病毒（IVV）方法。在这项研究中，我们进行了各种检查的PRMT 1底物，这是一个研究最多的酶在PRMT家族的模型筛选系统的建设。作为优化的结果，体外甲基化的模型底物在下拉实验中成功富集了11倍。

The purpose of this study was to construct an in vitro screening system for specific substrates of each PRMT based on an mRNA display technique, named in vitro virus (IVV) method. In this study, we performed various kinds of examination for construction of a model screening system for PRMT1 substrates, which is a most studied enzyme in the PRMT family. As a result of the optimization, an in vitro methylated model substrate was successfully enriched by 11-fold in a pull-down experiment.