Elucidation of the natural healing mechanism after knee anterior cruciate ligament tear and it's clinical application

用力学、组织学和免疫组织化学方法观察了前交叉韧带(ACL)断裂动物模型的自然愈合。用微型剪刀完全切断前交叉韧带，用人工韧带控制膝关节正常。所有大鼠前交叉韧带断裂均愈合。前交叉韧带切断8周后，愈合韧带中III型胶原增多。前交叉韧带切断后8周和40周，愈合韧带的强度仅为正常韧带的一半，内、外侧半月板和断裂前交叉韧带中的基质金属蛋白酶-1、基质金属蛋白酶-13和金属基质金属蛋白酶-1的表达增加。联合对照组与非联合对照组相比，基质金属蛋白酶-13的表达受到抑制。提示膝关节在重力作用下的异常运动增加，半月板蛋白水解酶和膝关节前交叉韧带断裂的蛋白水解酶表达增加。

The natural healing of the ruptured anterior cruciate ligament(ACL) using a new animal model was observed by mechanical, histological and immunohistochemistrical methods. The ACL was cut completely by the mini-scissors and then the knee joint was controlled normally with artificial ligament. The ruptured ACL was healed in all rats. Eight weeks after ACL cutting, the Type III collagen was increased in the healing ligament. After 8 weeks and 40 weeks after the ACL cut, the healing ligament was half strength compared to the normal ligament.The expression of MMP-1, MMP-13 and TIMP-1 in medial and lateral meniscus and rupture ACL was increased after ACL cut. Expression of MMP-13 in the joint control group has been suppressed compared to the no joint control group. It is suggested that abnormal joint movement under the gravity is increased to express of proteolytic enzymes of both meniscus and rupture ACL in the knee joint.