Analysis of stress-responsive non-coding antisense RNAs that are co-expressed with sense RNAs
Analysis of stress-responsive non-coding antisense RNAs that are co-expressed with sense RNAs
批准号:
21570056
负责人:
SEKI Motoaki
金额:
$3.0万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011
中文摘要
本研究在拟南芥中鉴定了约7000个逆境诱导反义RNA,并发现RD 29 A位点具有典型的干旱诱导基因,该位点具有一类与正义mRNA序列互补的逆境诱导反义RNA。真实的RT-PCR分析表明,RD 29 A反义RNA的积累在rdr 1/2/6三突变体中减少。我们的数据表明,RDR介导的反义RNA的合成在植物逆境条件下的正义mRNA的周转中起作用,这是一种新的RNA调节机制。
英文摘要
Our previous study identified about 7, 000 stress-inducible antisense RNAs in Arabidopsis and showed that RD29A locus that has typical drought-inducible gene, has a type of stress-inducible antisense RNA which is complementary to sequence of the sense mRNA. Real time RT-PCR analysis showed that accumulation of RD29A antisense RNA was decreased in rdr1/2/6 triple mutants. Our data indicates a novel RNA regulation mechanism that RDR-mediated synthesis of antisense RNAs functions in the turnover of sense mRNAs under the stress conditions in plants.
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RNA-dependent RNA polymeraseにより、環境ストレス下でアンチセンスRNAが生成する
RNA依赖性RNA聚合酶在环境压力下产生反义RNA
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[松井章浩, ら]
通讯作者:
ら
A survey and analysis of regulated alternative splicing events in Arabidopsis thaliana using genome tiling array and mRNA-seq
使用基因组平铺阵列和 mRNA-seq 对拟南芥中受调控的选择性剪接事件进行调查和分析
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Iida, K., Kawaguchi, S., Harada, E., Matsui, A., Seki, M. and Toyoda, T.]
通讯作者:
T.
DOI:
10.1093/pcp/pcq202
发表时间:
2011-02-01
期刊:
PLANT AND CELL PHYSIOLOGY
影响因子:
4.9
作者:
[Iida, Kei, Kawaguchi, Shuji, Toyoda, Tetsuro]
通讯作者:
Toyoda, Tetsuro
Functional genomics using RIKEN Arabidopsis thaliana Full-Length(RAFL)cDNAs
使用 RIKEN 拟南芥全长 (RAFL)cDNA 进行功能基因组学
DOI:
--
发表时间:
2009
期刊:
J.Plant Res. 122
影响因子:
--
作者:
[Seki, M.]
通讯作者:
M.
A computational elucidation of the transcriptional dynamics in Arabidopsis thaliana from comprehensive use of tiling array experiments
通过综合使用平铺阵列实验对拟南芥转录动力学进行计算阐明
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[Seki, M., Matsui A., Ishida J., Morosawa T., Iida K., Endo T., Okamoto M., Tanaka M., Kawaguchi S., Shinozaki K. and Toyoda T., 松島美喜子,松田勝, 川口修治]
通讯作者:
川口修治
共 26 条
Identification of novel siRNA pathways involved in plant environmental stress responses
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批准号:24657041
-
项目类别:Grant-in-Aid for Challenging Exploratory Research
-
资助金额:$2.58万
-
财政年份:2012
-
负责人:SEKI Motoaki
-
依托单位:
海外基金