Structural and molecular genetical analyses of factors related to bacterial chromosome condensation

我已经确定了细菌凝集素调节亚基(SCPA和SCPB)的功能区，并研究了它们的生化性质。此外，利用亚基的各种突变体，我检测了SMC亚基的ATPase活性。因此，SCPA的某些多肽区域通过与SCPB的C-末端结构域结合，控制SMC亚单位的ATPase活性。这一结果表明，调节亚复合体的内部构象变化导致细菌凝集素中SMC亚单位的二聚化。

I have identified functional regions of regulator subunits(ScpA and ScpB) of the bacterial condensin, and investigated their biochemical properties. In addition, using various mutants of the subunits, I examined the ATPase activity of the SMC subunit. As a result, some peptide regions of ScpA, through binding of the C-terminal domain of ScpB, control the ATPase activity of the SMC subunit. This result suggests that internal conformational changes of the regulatory subcomplex organize dimerization of the SMC subunit in the bacterial condensin.