Analysis of DG kinase functions related to PKC and its application for future drug.

阐明DGK的功能。在神经元中，我们发展了DGK。KO小鼠利用睡美人转座子系统，发现DGK。通过调节血脂来调节脊柱的形成，有助于维持神经网络在包括记忆和情绪在内的认知过程的突触传递中的作用。使用DGK。KO小鼠，我们研究了DGK的生理作用。在维生素E诱导的糖尿病肾功能障碍的改善中，发现DGK肾功能障碍。KO鼠病情严重，发病早于WT鼠。最后，我们发现c-Src使Tyr22和Tyr334磷酸化，而c-Abl使218Tyr磷酸化。

To elucidate function of DGK. in neurons, we developed DGK. KO mice using the Sleeping Beauty transposon system, and found that DGK. regulates spine formation by regulation of lipids, contributing to the maintenance of neural networks in synaptic transmission of cognitive processes including memory and emotion. Using DGK. KO mice, we investigated physiological role of DGK. in Vitamin E-induced improvement of diabetic renal dysfunction, and found that renal dysfunction of DGK. KO mice was sever and occurred earlier than WT. Finally, we discovered c-Src phosphorylated Tyr22 and Tyr334, while c-Abl phosphorylated 218Tyr.