Quantitative analysis of cell-cell interaction responsible for the robustness of development
Quantitative analysis of cell-cell interaction responsible for the robustness of development
批准号:
21570226
负责人:
YAMAMOTO Takashi
金额:
$3.0万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011
中文摘要
为了定量研究海胆胚胎发育过程中细胞间的相互作用,我们尝试建立了海胆胚胎中两个报告基因的定量体系。然而,我们不能观察到两个报告基因表达水平之间的关系。接下来,我们使用通过ZFN介导的基因添加产生的敲入胚胎分析了间充质细胞中的表达,发现依赖于细胞-细胞相互作用的变化,原代间充质细胞中的表达水平存在差异。
英文摘要
To quantitate the cell-cell interaction during development, we tried to establish the system for quantification of two reporter genes in sea urchin embryo. However, we could not observe the relation between the levels of two reporter gene expressions. Next we analyzed the expression in mesenchyme cells using knock-in embryos generated by ZFN-mediated gene addition, and found that there was variations in expression levels among primary mesenchyme cells dependent on the changes of cell-cell interaction.
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Self-organization of the phosph atidylinositol lipids ingnaling system for random cell migration
用于随机细胞迁移的磷脂酰肌醇脂质信号系统的自组织
DOI:
--
发表时间:
2010
期刊:
Proc Natl Acad Sci USA
影响因子:
--
作者:
[Arai Y, Shibata T, Matsuoka S, Sato MJ, Yanagida T, Ueda M.]
通讯作者:
Ueda M.
Genetic manipulation in seaurchin embryo using zinc-finger nucleases
使用锌指核酸酶对海胆胚胎进行遗传操作
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[三浦郁夫, 大谷浩己, 尾形光昭, Ochiai Hiroshi]
通讯作者:
Ochiai Hiroshi
DOI:
10.1002/dvdy.22074
发表时间:
2009-10-01
期刊:
DEVELOPMENTAL DYNAMICS
影响因子:
2.5
作者:
[Fujii, Takayoshi, Sakamoto, Naoaki, Yamamoto, Takashi]
通讯作者:
Yamamoto, Takashi
適応反応のゆらぎと応答,理研シンポジウム「細胞システムの動態と論理II」
适应性反应的波动与响应,理化学研究所研讨会“细胞系统的动力学与逻辑II”
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Minegishi H, Kamekura M, Kitajima-Ihara T, Nakasone K, Echigo A, Shimane Y, Usami R, Itoh T, Ihara K, 西川正俊]
通讯作者:
西川正俊
Deterministic and probabilistic aspects of signal processing and transduction in chemotactic response of eukaryotic cells
真核细胞趋化反应中信号处理和转导的确定性和概率方面
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[Kaburaki T, Takamoto M, Numaga J, Kawashima H, Araie M, Ohnogi Y, Harihara S, Kuwata S, Takeuchi F, 藤田和将, 柴田達夫]
通讯作者:
柴田達夫
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