Studies on the infection mechanisms of Strongyloides venezelensis by analyzing genes expressed in infective larvae.

采用常规EST分析和下一代焦磷酸测序仪大规模测序的方法，对啮齿类肠道线虫委内瑞拉圆线虫感染幼虫的基因表达进行了研究。近一半的基因在感染幼虫期特异性表达，编码功能未知的新蛋白质，但也检测到了几个astacin样金属蛋白酶基因。这些锌金属蛋白酶可以解释感染性幼虫在感染后的皮肤侵入。Astacin样金属蛋白酶似乎形成一个大的基因家族，表明其在寄生性中的重要性。

Gene expression of infective larvae of Strongyloides venezuelensis, an intestinal nematode of rodents, was examined by means of conventional EST analysis and a large-scale sequencing with a next generation pyrosequencer. Nearly half of the genes which were expressed specifically in the infective larva stage coded novel proteins of unknown function, though several genes for astacin-like metalloprotease were detected. These zinc-metalloproteases could account for the skin invasion of infective larvae upon infection. Astacin-like metalloproteases appeared to form a large gene family, suggesting its importance in parsitism.