Analysis of nonalcoholic fatty liver using androgen receptor transgenic mouse

我们曾计划使用雄激素受体转基因小鼠来研究雄激素受体与脂质或碳水化合物代谢的关系，但未能生产出可用于研究的转基因小鼠。在第一个实验中产生的两只雌性转基因小鼠没有抚养它们的孩子。在接下来的3个连续实验中产生的总共139只小鼠没有转基因基因。因此，我们将雄激素受体表达载体分别转染HepG2和Huh7细胞，分析了84个脂代谢相关基因mRNA表达的变化。在这些基因中，在这两种细胞中唯一下调的基因是酰基辅酶A脱氢酶和肉毒碱棕榈酰转移酶1A，这两个基因都参与脂肪酸氧化，结果与我们的预期相反。

We had planned to examine the relationship between androgen receptor and lipid or carbohydrate metabolism using androgen receptor transgenic mouse, but the transgenic mouse could not be produced for examination. Two female transgenic mice produced in the first experiment did not raise their children. A total of 139 mice produced in the next 3 consecutive experiments did not have the transgenic genes. Therefore we transfected androgen receptor expression vector into HepG2 and Huh7, and analyzed the change of m-RNA expression in the 84 genes related to lipid metabolism. Among these genes, the only genes down regulated both in the two cells were Acyl-CoA dehydrogenase and Carnitine palmitoyltransferase 1A, both of which are involved in fatty acid oxidation and the result was contrary to our expectation.