The effects of early maternal separation followed by social deprivation on the development and function of GABAergic system in the limbic structures

为了验证新生儿母亲剥夺社会隔离的社会应激影响边缘结构中特定GABA能中间神经元亚类的社会行为相关生理功能的假设，研究了Wistar大鼠早期应激相关的钙结合蛋白表达水平、社会行为和自发抑制性突触后电流（sIPSC）活性的变化。早期社会应激可使基底外侧杏仁核和内侧前额叶皮质II /III层的小清蛋白（PV）表达水平升高，而边缘结构的钙网膜蛋白（CR）和钙结合蛋白（CB）表达水平无明显变化。肛门嗅闻行为，社会互动测试的一个测试，倾向于增加在压力下的动物。然而，通过从边缘区的主要神经元记录的sIPSC的平均振幅和频率评估的GABA能中间神经元的电生理测量在应激组和对照非应激组之间没有显示出任何差异。这些结果表明，早期社会应激通过GABA能中间神经元以外的机制产生改变的社会行为

To test the hypothesis that social stress of neonatal maternal deprivation followed by social isolation affects social behavior-relevant physiological functions of specific subclasses of GABAergic interneurons in the limbic structures, early stress-related changes in the expression levels of calcium binding proteins, social behaviors and the activity of spontaneous inhibitory postsynaptic currents (sIPSCs) were investigated for Wistar rats. Early social stress was found to elevate the expression levels of parvalbumin (PV) in basolateral amygdala and II /III layers of medial prefrontal cortex, whereas those of calretinin (CR) and calbindin (CB) showed no changes in the limbic structures. Ano-genital sniffing behavior, one test for social interaction test, tended to increase in the neonatally stressed animals. Electrophysiological measurements of GABAergic interneurons assessed by the mean amplitude and the frequency of sIPSCs recorded from principal neurons in the limbic regions, however, did not show any difference between the stressed group and control non-stressed group. These results suggest that early social stress produces altered social behavior via mechanisms other than those involving GABAergic interneurons