Functionalization of microbes cultured in 2D on the newly developed optical feedback system

我们开发了一种新的光反应微生物微流控装置的光反馈系统。利用绿藻的疏光反应，成功地实现了微水族箱中细胞密度的任意模式动态控制。在反馈算法中引入神经网络算法，实现了神经计算，实现了动态过渡的优化解。此外，利用微生物的趋化性，我们成功地在两个单独的培养系统之间建立了光学反馈和化学传感的人工连接。

We developed a new optical feedback system for the photoreactive microbes confined in a microfluidic device. By using the photophobic reaction of Euglena, we succeeded in the control of cell density in the micro-aquarium dynamically with arbitrary pattern. We also achieved neuro-computing by introducing neural network algorithm in the feedback algorithm, by which optimized solution can be obtained with dynamic transition. Further, we succeeded in artificial connection between two individual culture system with optical feedback as well as chemical sensing using chemotaxis of the microbes.