Analysis of intraspecific diversification caused by phytochrome gene evolution as photo-receptor

我们的目的是评估光感受器基因多态性，如光敏色素和隐色素与光周期响应的关系，作为局部适应栖息地环境。首先，我们完成了日本小豆蔻（Cardamine nipponica，芸苔科）PHYE比较生理研究的基础构建，其中小豆蔻在日本列岛呈现南北分化。我们分离到了PHYE的北部和南部基因型，并成功克隆了该基因的N'端部分。其次，分析了日本莲光感受器基因PHY和CRY的基因多态性和地理结构。该植物在PHYA、PHYB和PHYE中含有多个snp，其中大部分位于GAF环等功能域。

We aimed to evaluate polymorphisms of photoreceptor genes such as phytochromes and cryptochromes in relation with photoperiodic response as local adaptation to habitat environment.First, we accomplished to construct basement for comparative physiological studies on PHYE of Cardamine nipponica(Brassicaceae), of which exhibits north-south differentiation in the Japanese archipelago. We isolated the northern and southern PHYE genotypes, and succeeded in cloning the N'-terminal part of the gene.Second, we analyzed gene polymorphisms and geographical structure of photoreceptor genes such as PHY and CRY of Lotus japonicus. This plant harbored plural SNPs in PHYA, PHYB and PHYE, of which most of them were located in functional domains such as GAF loop.