Evaluation of Acetobacter spp. as a novel inoculant to improve silage management

从未处理的玉米青贮饲料中分离到巴氏醋酸杆菌为优势菌。通过变性梯度凝胶电泳，以及包括中国北京在内的不同地区生产的其他玉米青贮饲料中证实了巴氏杆菌的存在。利用该分离物，测定了巴氏杆菌对发酵、好氧稳定性和细菌群落的影响。青贮前添加巴氏巴氏杆菌提高了玉米的好氧稳定性，但对禾草和水稻青贮没有效果，而在开仓后添加巴氏巴氏巴氏杆菌加速了玉米青贮的好氧腐败。这些结果表明，巴氏杆菌可以寄生在玉米青贮饲料中，并可能与其他与厌氧储存和好氧变质有关的细菌物种发生显著的相互作用。

Acetobacter pasteurianus was isolated from bunker-made untreated corn silage as the predominant bacterium. The presence of A. pasteurianus was confirmed by denaturing gradient gel electrophoresis, and in other bunker-made corn silages produced in different regions including Beijing, China. Using the isolate, the effects of A. pasteurianus on fermentation, aerobic stability and bacterial community were determined. Addition of A. pasteurianus before ensiling improved aerobic stability in corn but not in grass and rice silage, whereas addition of A. pasteurianus after silo opening accelerated aerobic spoilage in corn silage. These results indicate that A. pasteurianus can inhabit bunker-made corn silage, and may show significant interactions with other bacterial species associated with anaerobic storage and aerobic deterioration.