Evaluation of nutritional significance and microbial production of 1, 2-propanediol in silage

青贮饲料中1, 2-丙二醇的营养意义和微生物生产评价

• 批准号: 13660267
• 负责人: NISHINO Naoki
• 金额: $ 2.62万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660267/
• 关键词: Feed; Lactic acid bacteria; Silage

This research was aimed to identify microbial factors responsible for 1, 2-propanediol formation in silage. The value of dietary 1, 2-propanediol in ruminants was also investigated.From a mixed silage primarily containing wet brewers grains, a 1, 2-propanediol producing microorganism was isolated. This gram-positive, catalase-negative and heterofermentative lactic acid bacteria was identified as Lactobacillus buchneri. When L. buchneri was inoculated to whole-crop maize and grasses, significant amounts of 1, 2-propanediol were produced in the silages. The production was coupled with a decrease in lactate and an increase in acetate, and was enhanced when storage period was prolonged The highest content was 77 g/kg DM in this study.Aerobic stability of silages was considerably improved when L. buchneri was inoculated, probably because the increased acetate could act as an antimicrobial component and lower yeast population during ensilage.Exogenous 1, 2-propanediol was degraded by rumen microorganisms and the ratio of acetate to propionate decreased, while not affecting the intake and preference of total mixed ration. Also, the intake of total mixed ration was not altered, when L. buchneri inoculated silage was incorporated.It is concluded that L. buchneri can be a major 1, 2-propanediol producing microorganism in silage and could be used as a novel additive to inhibit aerobic deterioration. The intake and preference of diets would be unaffected by 1, 2-propanediol, when inoculated silages were offered as ingredients of total mixed ration.

本研究旨在确定影响青贮饲料中1，2-丙二醇形成的微生物因素。从以湿啤酒糟为主要原料的混合青贮饲料中分离到一株1，2-丙二醇产生菌。这种革兰氏阳性、过氧化氢酶阴性且异型发酵的乳酸菌被鉴定为布氏乳杆菌（Lactobacillus buchneri）。当L.将Buchneri接种到整株玉米和草中，在青贮饲料中产生了显著量的1，2-丙二醇。随着贮藏时间的延长，乳酸的产量增加，乙酸的产量增加，乳酸的产量降低，最高可达77 g/kg DM。接种布赫内里，可能是因为增加的醋酸盐可以作为抗微生物成分，降低青贮过程中的酵母菌群。外源1，2-丙二醇被瘤胃微生物降解，醋酸盐与丙酸盐的比例下降，但不影响全混合日粮的摄入量和偏好。当L.结果表明，L. Buchneri是青贮饲料中主要的1，2-丙二醇产生菌，可作为一种新型添加剂来抑制好氧变质。以接种青贮饲料作为全混合日粮成分时，1，2-丙二醇对日粮的采食量和选择性没有影响。

项目成果

Nishino, N., Harada, H. and Sakaguchi, E.: "Evaluation of fermentation and aerobic stability of wet brewers grains ensiled alone or in combination with various dry feeds as a total mixed ration"Journal of the Science of Food and Agriculture. 94. 800-807 (

Nishino, N.、Harada, H. 和 Sakaguchi, E.：“单独青贮的湿啤酒谷物或与各种干饲料组合作为总混合日粮的发酵和有氧稳定性的评估”食品和农业科学杂志。

Nishino, N., Yoshida, M., Shiota, H., Sakaguchi, E.: "Accumulation of 1,2-propanediol and enhancement of aerobic stability in whole crop maize silage inoculated with Lactobacillus buchneri"Journal of Applied Microbiology. 83. 557-563 (2003)

Nishino, N.、Yoshida, M.、Shiota, H.、Sakaguchi, E.：“接种布氏乳杆菌的整株玉米青贮饲料中 1,2-丙二醇的积累和有氧稳定性的增强”应用微生物学杂志。