Identification of membrane proteins associated with Prion invasion in bovine intestinal microfold (M)cells

为了鉴定与朊病毒侵袭牛肠微褶(M)细胞相关的膜蛋白，用重组牛朊病毒蛋白包被磁珠处理M细胞分化的牛肠上皮细胞（BIE细胞），并从BIE细胞中纯化出含磁珠的囊泡。双向电泳后，采用LC-MS/MS谱仪鉴定果糖二磷酸醛缩酶A。醛缩酶A在回肠Peyer’s patches的M细胞和分枝杆菌咽上皮上特异性表达，这些组织被认为与朊病毒侵袭有关。此外，远西分析显示醛缩酶A可以与朊病毒蛋白结合。

In order to identify a membrane protein associated with the Prion invasion in bovine intestinal microfold (M)cells, M cell-differentiated bovine intestinal epitheliocyte cell line (BIE cells)were treated with recombinant bovine prion protein-coated magnetic beads, and then vesicles including magnetic beads were purified from BIE cells. After 2D-electrophoresis, fructose-bisphosphate aldolase A was identified using LC-MS/MS spectrometer. Aldolase A were specifically expressed on M cells in ileal Peyer's patches and the epithelium in myctero pharynges, these tissues of which were thought to be associated with Prion invasion. In addition, the far western analysis revealed that aldolase A could bind to prion protein.