The establishment of new marker to diagnose the wound age

RNA分析已应用于法医工作，以确定伤口的年龄。我们已经使用定量RT-PCR研究了10个基因的mRNA表达，包括c-fos，fosB，丝裂原活化蛋白激酶磷酸酶-1（MKP-1），CD 14，趋化因子（C-C基序）配体9（CCL 9）、胎盘生长因子（PlGF）、肥大细胞蛋白酶-5（MCP-5）、生长停滞特异性5（Gas 5）、β-2微球蛋白（B2 M）和主要尿蛋白-1（MUP-1），在创伤年龄的成年小鼠的修复反应方面。在本研究中，我们重点研究了创伤愈合过程中CD 14蛋白的表达。免疫组化和western blot检测结果显示，创伤后3 ~ 5天，CD 14蛋白表达增加。此外，CD 14特异性表达于伤口周围的巨噬细胞。结果表明，检测CD 14蛋白对法医学上推断创伤时间有一定的参考价值。

RNA analysis has been applied to forensic work to determine wound age. We already investigated mRNA expression using quantitative RT-PCR of ten genes, including c-fos, fosB, mitogen-activated protein kinase phosphatase-1(MKP-1), CD14, chemokine(C-C motif) ligand 9(CCL9), placenta growth factor(PlGF), mast cell protease-5(MCP-5), growth arrest specific 5(Gas5), beta-2 microglobulin(B2M) and major urinary protein-1(MUP-1), in terms of repair response in adult mice of wound age. In this study, we focused on the CD14 protein expression during wound healing. We demonstrated that the CD14 protein only expressed from 3 to 5 day after wound with immunohistological and western blot method. In addition, the CD14 specifically expressed at macrophage around wound. These results indicated that investigation of CD 14 protein must be useful of estimation for wound age in forensic practice.