Novel approach of induction of donor specific immune tolerance: adoptive transfer of Treg cells combined with MF1 gene transfection

我们开发了一种新型的高效基因转染技术，使用慢病毒载体结合超声介导的基因转染程序。我们已经成功地将含有HGF和MSP选定功能域的工程嵌合因子metron factor基因转染到移植肾组织中。局部产生的MF-1，可能会增强Treg细胞在体内的扩增，结合。在我们的大鼠肾移植模型中，Treg细胞的过继性转移成功地诱导了超过70%的供体特异性免疫耐受。

We have developed a novel approach of highly effective gene transfection technique using lenti-virus vector combined with ultrasound mediated gene transfection procedure. We have performed successful gene transfection of metron factor, which is the engineered chimeric factors containing selected functional domains of HGF and MSP, into the transplanted kidney tissue. Local production of MF-1, which may enhance the expansion of Treg cells in vivo, combined with .the adoptive transfer of Treg cells, successfully induced the donor specific immune tolerance in more than 70% of recipients in our rat kidney transplant model.