a Novel Screening System for Potential Anticancer Drugs with Gene Amplification Method

DNA结合分子是一种潜在的调节基因扩增或基因表达的抗癌药物。化合物DNA结合亲和力的准确相对测定是药物发现领域的一个重要因素。在这项工作中，我开发了一种新的系统，使用实时聚合酶链式反应分析仪来评估具有底物-二聚体(PD)模板的化合物的相对DNA结合亲和力。PD模板的使用使我们能够准确和高灵敏地测定化合物的DNA结合亲和力，而不需要扩增非特异的PCR产物。我们还结合上述金纳米粒子聚集现象和基于聚合酶链式反应的评价系统，开发了化合物DNA结合亲和力的比色测定系统。

DNA-binding molecules are potential anticancer drugs to regulate gene amplification or gene expressions. The accurate relative determination of DNA-binding affinities of chemical compounds is an important factor for drug discovery area. In this work, I have developed a novel system to evaluate relative DNA-binding affinities of chemical compounds with primer-dimer(PD) templates using a real-time PCR analyzer. The utility of PD templates allows us to be accurate and highly sensitive determination of DNA-binding affinities of chemical compounds without the amplification of non-specific PCR products. We also developed the colorimetric determination system of DNA-binding affinities of chemical compounds in combination with gold nanoparticle aggregation phenomena and PCR-based evaluation system described above.