Functional analysis of the cell cycle factors in the alveolar bone remodeling by the orthodontic force

据报道，细胞周期因子在骨生成和骨吸收中都起着重要作用。本研究的目的是：(1)研究正畸力作用下牙槽骨成骨细胞和破骨细胞细胞细胞周期因子表达模式的变化；(2)通过调节细胞周期因子来开发一种新的正畸治疗方法。首先，建立了正畸力作用下牙齿移动的实验模型。其次，我尝试研究正畸力刺激下成骨细胞细胞周期因子基因表达模式的变化。为此，我们利用DNA微阵列分析了在成骨细胞分化促进过程中表达变化的细胞周期因子。因此，一些转录因子的表达水平发生了变化，这些转录因子在成骨过程中的作用以前没有报道过。此外，cyclin B1、cyclin E1和p21表达增加，p57表达减少。

It has been reported that cell cycle factors play important roles in both bone generation and bone resorption. The aim of this study was (1) to investigate the change of expression patterns of cell cycle factors in osteoblasts and osteoclasts in alveolar bone, where orthodontic force was applied and (2) to develop a new orthodontic treatment method by regulating cell cycle factors. First, an experimental model of tooth movement by orthodontic force was established. Second, I tried to investigate change of gene expression patterns of cell cycle factors in osteoblasts stimulated by orthodontic force. For this purpose, DNA micro array was performed to analyze cell cycle factors of which expression changed when osteoblast differentiation was promoted. Consequently, the expression levels of some transcription factors changed, whose role in osteogenesis had not been reported before. In addition, it was revealed that expressions of cyclin B1, cyclin E1 and p21 increased, and that of p57 decreased.