Receptor-mediated cell-cell communication in plants: functional analysis of the LRR-V/SRS family of putative leucine-rich repeat receptor-like kinase in Arabidopsis thaliana

众所周知，植物细胞必须反复评估和交流它们的相对位置，以允许适当的发育发生。然而，我们对细胞间通讯的潜在机制知之甚少。该项目为长期探索拟南芥中新的受体激酶依赖信号转导通路提供了基础。因此，这也将提高我们对植物中受体介导的信号转导的认识。该资助提案基于我们之前对STRUBBELIG （SUB）的分析结果，该基因编码一种新型富亮氨酸重复受体样激酶（LRR-RLK）。遗传分析表明，sub1基因在茎尖分生组织的发育、茎尖分生组织的大小、花器官的形成和外生以及被皮形态发生等方面都是必需的。因此，SUB定义了一种新的基于受体激酶的信号转导途径，对器官发生具有普遍重要性。SUB和8个功能未知的同源物属于拟南芥LRR-RLKs家族。建议的研究将基于反向遗传学，细胞和分子生物学以及生物化学的方法。主要的重点将停留在分子表征和八个亚同源家族的生物学功能的研究。还计划进行实验，以解决蛋白质的细胞内定位及其潜在的相互作用。

It is well established that plant cells must repeatedly assess and communicate their relative positions in order to allow proper development to occur. Little is known, however, about the underlying mechanisms of cell-cell communication. This project provides the basis for a long-term exploration of novel receptor kinase-dependent signal transduction pathways in Arabidopsis thaliana. It will thus also enhance our knowledge on receptor-mediated signal transduction in plants in general. The grant proposal is based on results from our previous analysis of STRUBBELIG (SUB), a gene encoding a novel type of leucinerich repeat receptor-like kinase (LRR-RLK). Genetic analysis indicated that SUB is required for apical meristem development, apical meristem size, floral organ initiation and outgrowth, and integument morphogenesis. Thus, SUB defines a novel receptor kinase-based signal transduction pathway of general importance for organogenesis. SUB and eight homologs of unknown function belong to the Arabidopsis LRR-V family of LRR-RLKs. The proposed research will be based on methods of reverse genetics, cellular and molecular biology, and biochemistry. The major focus will reside on the molecular characterization and the investigation of the biological function of the eight SUB homologs of the family. Experiments are also planned that address the intracellular localization of the proteins and their potential interactions.